2004 Oct;14(10B):2121-7. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Interacts with the complement complex. , CLU has been shown to interact with Ku70. [, Ramachandran P, Boontheung P, Xie Y, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . Although the etiology is unknown, â¦ 2007 Jan 26;282(4):2278-87. [, Picariello G, Ferranti P, Mamone G, Roepstorff P, Addeo F: Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry. Appl Theor Electrophor. Interacts with the complement complex. J Proteome Res. These two subunits are subsequently linked by five disulphide bonds to form an anti-parallel heterodimer [ 39 ]. The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. All buffers and samples were filtered (0.45 m) before use. Eur J Biochem. 2003 Mar 28;278(13):11590-600. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa). Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). Mol Cell Proteomics. It is encoded by one single gene and cleaved posttranslationally into Î± (34-36 kDa) and Î² â¦ The first of these criteria was satisfied by a recent report that clusterin forms high-molecular-weight âsolubilizedâ complexes with heat- or reduction-stressed proteins, inhibiting their precipitation. [, de Silva HV, Stuart WD, Park YB, Mao SJ, Gil CM, Wetterau JR, Busch SJ, Harmony JA: Purification and characterization of apolipoprotein J. J Biol Chem. , Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. 2006 Jan 19;439(7074):331-5. J Cell Physiol. 2007 May 25;369(1):157-67. Apomixis is a naturally occurring mode of reproduction that results in embryo formation without the involvement of meiosis or fertilization of the egg. 1991 Oct 1;88(19):8577-81. The structure of clusterin is predicted to contain amphipathic helices, which may facilitate its association with HDL particles [122, 125]. [, Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. Epub 2013 Nov 22. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. 2007 Sep;1772(9):1103-11. [, Kumita JR, Poon S, Caddy GL, Hagan CL, Dumoulin M, Yerbury JJ, Stewart EM, Robinson CV, Wilson MR, Dobson CM: The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species. The zeta potentials of all three nanoparticles were approximately -20 mV (Figure S5C). 2007 Jun;6(6):1039-48. [, Ehnholm C, Bozas SE, Tenkanen H, Kirszbaum L, Metso J, Murphy B, Walker ID: The apolipoprotein A-I binding protein of placenta and the SP-40,40 protein of human blood are different proteins which both bind to apolipoprotein A-I. , The CLU gene contains nine exons and expresses three isoforms alternatively-spliced at the first exon. [, Danik M, Chabot JG, Mercier C, Benabid AL, Chauvin C, Quirion R, Suh M: Human gliomas and epileptic foci express high levels of a mRNA related to rat testicular sulfated glycoprotein 2, a purported marker of cell death. Eur J Biochem. Clusterin has been shown to stain 95% of systemic ALCL, including 100% of ALK-1(+) and 91% of ALK-1(-) ALCL. [, Kirszbaum L, Sharpe JA, Murphy B, d'Apice AJ, Classon B, Hudson P, Walker ID: Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems.  They also perform opposing functions: the nuclear CLU binds Ku70 to release BAX and induce apoptosis, whereas the cytosolic and secretory isoforms inhibit apoptosis. Furthermore, in the absence of the leader sequence, the expressed clusterin had a molecular weight consistent with that of the predicted holoprotein (40 kDa), suggesting a compromised postâtranslational processing with diffuse distribution throughout the cytoplasm. 2009 May;219(2):314-23. doi: 10.1002/jcp.21671. [PubMed:8181474] J Proteome Res. Nat Biotechnol. Proc Natl Acad Sci U S A. FEBS Lett. [, Kirszbaum L, Bozas SE, Walker ID: SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. High-molecular complexes displaying clusterin associated with the soluble PrP were also found in male seminal fluid . Due to an increasing aging population, this number is predicted to rise to over 130 million cases by 2050 (Prince et al., 2015; Alzheimer Association, 2016). Forms high-molecular weight oligomers upon interaction with misfolded proteins. 2009 Feb;8(2):651-61. doi: 10.1021/pr8008012. In humans, clusterin is encoded by the CLU gene on chromosome 8. [, Zhang H, Kim JK, Edwards CA, Xu Z, Taichman R, Wang CY: Clusterin inhibits apoptosis by interacting with activated Bax. 2009 Nov;6(11):809-11. doi: 10.1038/nmeth.1392. [, Nilsson J, Ruetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G: Enrichment of glycopeptides for glycan structure and attachment site identification. BMC Genomics. Humphreys D. et al. Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. [, Ghiso J, Matsubara E, Koudinov A, Choi-Miura NH, Tomita M, Wisniewski T, Frangione B: The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex. 2006 Jun;5(6):1493-503. Clusterin (CLU), or apolipoprotein J (ApoJ), is the third most predominant genetic risk factor associated with late-onset Alzheimerâs disease (LOAD).  The gene is highly conserved in species, and the protein is widely distributed in many tissues and organs, where it participates in a number of biological processes, including lipid transport, membrane recycling, cell adhesion, programmed cell death, and complement-mediated cell lysis. Not yet tested in other applications. Unlike most other chaperone proteins, which aid intracellular proteins, CLU is a Golgi chaperone that facilitates the folding of secreted proteins in an ATP-independent way. Mol Immunol. Identity with SP-40,40, an inhibitor of complement-mediated cytolysis. However, little is known about the specific mechanisms that act to suppress this process in vivo. Slightly acidic pH promotes interaction with misfolded proteins. Epub 2007 Jun 13.  CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. Clusterin has been suggested to have a protective role during â¦ 2006 Apr;8(4):565-80. [, Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. 1992 Oct;112(4):557-61. [, Ranney MK, Ahmed IS, Potts KR, Craven RJ: Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer. The Mr for dimeric RTF clusterin was CCSt found to be 94 kDa; however, the more accurate estimate (1 hour by reduced conditions suggested that sum of the â¦ No. 1989 Sep;86(18):7123-7. Seed-derived progeny of an apomictic plant are genetically identical to the maternal parent. Nat Cell Biol. Proteintech Anti-Clusterin Polyclonal, Catalog # 12289-1-AP. Forms high-molecular weight oligomers upon interaction with misfolded proteins. 2002 Oct 18;277(42):39532-40. Choose a Store Santa cruz biotechnology. 2002 Jun;269(11):2789-94.  In addition to the above diseases, CLU has been linked to other conditions resulting from oxidative damage, including aging, glomerulonephritis, atherosclerosis, and myocardial infarction. [, Kapron JT, Hilliard GM, Lakins JN, Tenniswood MP, West KA, Carr SA, Crabb JW: Identification and characterization of glycosylation sites in human serum clusterin. Background Information. 14. Biochim Biophys Acta. [, Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson CC: Differential regulation of clusterin and its isoforms by androgens in prostate cells. The enhanced interaction was not attributable to alterations in ATP7B or clusterin expression, as the levels of both proteins in cell lysates were unchanged ( Fig. Unlike most other cancers, which feature upregulated CLU levels to enhance tumor cell survival, testicular seminoma features downregulated CLU levels, allowing for increased sensitivity to chemotherapy treatments. The integrity of the complexes was checked peri-odically by reanalysis using the same column. 2011 Dec;68(23):3919-31. doi: 10.1007/s00018-011-0684-8). Biochim Biophys Acta. Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). Epub 2005 Aug 21. [, Nizard P, Tetley S, Le Drean Y, Watrin T, Le Goff P, Wilson MR, Michel D: Stress-induced retrotranslocation of clusterin/ApoJ into the cytosol. , CLU is a member of the small heat shock protein family and, thus, a molecular chaperone. [, Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, Taudien S, Garber M, Kodira CD, Schueler MG, Shimizu A, Whittaker CA, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Yang X, Allen NR, Anderson S, Asakawa T, Blechschmidt K, Bloom T, Borowsky ML, Butler J, Cook A, Corum B, DeArellano K, DeCaprio D, Dooley KT, Dorris L 3rd, Engels R, Glockner G, Hafez N, Hagopian DS, Hall JL, Ishikawa SK, Jaffe DB, Kamat A, Kudoh J, Lehmann R, Lokitsang T, Macdonald P, Major JE, Matthews CD, Mauceli E, Menzel U, Mihalev AH, Minoshima S, Murayama Y, Naylor JW, Nicol R, Nguyen C, O'Leary SB, O'Neill K, Parker SC, Polley A, Raymond CK, Reichwald K, Rodriguez J, Sasaki T, Schilhabel M, Siddiqui R, Smith CL, Sneddon TP, Talamas JA, Tenzin P, Topham K, Venkataraman V, Wen G, Yamazaki S, Young SK, Zeng Q, Zimmer AR, Rosenthal A, Birren BW, Platzer M, Shimizu N, Lander ES: DNA sequence and analysis of human chromosome 8. 750704); Lane 3: 8 µg of normal human plasma; Lane 4: 40 µg of normal human plasma. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Nat Genet.  In humans, clusterin is encoded by the CLU gene on chromosome 8. Studies have shown that clusterin may be a useful diagnostic marker for ALCL, especially in ALK-1(-) cases. Product is not sterile! After further adsorption with CLU, the particle size was 151.8 nm. [, Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state. In clear cell renal cell carcinoma, CLU functions to regulate ERK 1/2 signaling and matrix metallopeptidase-9 expression to promote tumor cell migration, invasion, and metastasis. [, Yerbury JJ, Poon S, Meehan S, Thompson B, Kumita JR, Dobson CM, Wilson MR: The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures. [, Zhang H, Li XJ, Martin DB, Aebersold R: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Proteomics. PEO with a weightâaverage molecular weight (M w) of 100,000 g/mol and a numberâaverage molecular weight (M n) of 96,000 g/mol was dissolved in freshly doubleâdistilled deionized (DI) water (pH â¼ 6.4) at a concentration of approximately 0.025 wt %. J Proteomics. CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes.  In particular, custirsen, an antisense oligonucleotide that blocks the CLU mRNA transcript, enhanced heat-shock protein 90 (HSP90) inhibitor activity by suppressing the heat-shock response in castrate-resistant prostate cancer, and is currently in phase III trials. CLU may promote tumorigenesis by facilitating BAX-KLU70 binding and, consequently, preventing BAX from localizing to the outer mitochondrial membrane to stimulate cell death. molecular weight calibration kit (GE Healthcare). Alzheimerâs disease (AD) is the most common form of dementia, accounting for over 60% of the 46.8 million cases worldwide. Such increase was related to the increase of molecular weight of enzymes. Supplied as 150 µL purified antibody (0.2 mg/mL). , spherical high-density lipoprotein particle, central nervous system myelin maintenance, negative regulation of protein homooligomerization, chaperone-mediated protein complex assembly, positive regulation of tau-protein kinase activity, negative regulation of amyloid-beta formation, positive regulation of nitric oxide biosynthetic process, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, positive regulation of neurofibrillary tangle assembly, positive regulation of amyloid-beta formation, regulation of neuronal signal transduction, positive regulation of NF-kappaB transcription factor activity, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tumor necrosis factor production, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, release of cytochrome c from mitochondria, extracellularular signal-regulated kinase, GRCh38: Ensembl release 89: ENSG00000120885, GRCm38: Ensembl release 89: ENSMUSG00000022037, "Clusterin: a key player in cancer chemoresistance and its inhibition", "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis", "Ram rete testis fluid contains a protein (clusterin) which influences cell-cell interactions in vitro", "Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer's disease", "Plasma clusterin and the risk of Alzheimer disease", "Plasma Protein Appears to Be Associated With Development and Severity of Alzheimer's Disease", "Exploring functional candidate genes for genetic association in german patients with pseudoexfoliation syndrome and pseudoexfoliation glaucoma", "Risk of non-Hodgkin lymphoma in association with germline variation in complement genes", "Roles of clusterin in progression, chemoresistance and metastasis of human ovarian cancer", "Clusterin expression in cutaneous CD30-positive lymphoproliferative disorders and their histologic simulants", "Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression", "Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex", "Clusterin expression correlates with stage and presence of large cells in mycosis fungoides", "Hepatitis delta virus epigenetically enhances clusterin expression via histone acetylation in human hepatocellular carcinoma cells", "Challenge and promise: roles for clusterin in pathogenesis, progression and therapy of cancer", "Genome-wide association study of panic disorder in the Japanese population", https://en.wikipedia.org/w/index.php?title=Clusterin&oldid=997744631, Creative Commons Attribution-ShareAlike License, This page was last edited on 2 January 2021, at 01:07. J Biol Chem. J Biol Chem. Clusterin (CLU) is a gene/protein highly conserved and expressed in several human tissues and fluids. 1994 May 1;221(3):917-25. The blot was incubated with â¦ Epub 2005 Oct 31. 2009 May;8(5):913-23. doi: 10.1074/mcp.M800504-MCP200. [, Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. [, Santilli G, Aronow BJ, Sala A: Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity. [, Rizzi F, Caccamo AE, Belloni L, Bettuzzi S: Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells. Proteomics. Distribution Tissue distribution: Ubiquitously expressed. 2005 Sep;7(9):909-15. Theoretical Molecular Weight: 52495: Formulation: Human native Clusterin was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.1M phosphate buffer, 0.15M NaCl pH 7.5. Epub 2009 Oct 18. Biol Reprod. Nature. 1 , C and D ). [, Wang Z, Widgren EE, Richardson RT, O'Rand MG: Characterization of an eppin protein complex from human semen and spermatozoa. The chaperone protein then aids hepatitis C viral assembly by stabilizing its core and NS5A units. Mol Cancer Res. Interacts with APOA1, LRP2, CLUAP1 and PON1. 2007 Aug;21(10):2312-22.  The mature protein is a 449-residue, heterodimeric, disulfide-linked glycoprotein composed of two subunits of 40 kDa Î±- and Î²-chains. The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . 1988;1(1):73-6. 2004 Feb;4(2):454-65. EMBO J. [, Bunkenborg J, Pilch BJ, Podtelejnikov AV, Wisniewski JR: Screening for N-glycosylated proteins by liquid chromatography mass spectrometry. Genome Res. Epub 2009 Jun 17. Epub 2003 May 18. Elastin and clusterin were detected by fluorescence at an excitation wavelength of 275 nm and an emission wavelength of 307 nm using a FP 920 fluorescence detector (Jasco, Grossumstadt, Germany). Other cancers CLU has been implicated in include breast cancer, pancreatic cancer, hepatocellular carcinoma, and melanoma. Clusterin has chaperone-like activity similar to that of small heat-shock proteins. FASEB J. Oncogene. [, Liu T, Qian WJ, Gritsenko MA, Camp DG 2nd, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.  In addition to Alzheimerâs disease, CLU is involved in other neurodegenerative diseases such as Huntington disease.. Cell Microbiol. Standard proteins from the molecular weight marker kit (aldolase, ferritin, and bovine serum albumin; Sigma) were used for calibration. [, Stewart EM, Aquilina JA, Easterbrook-Smith SB, Murphy-Durland D, Jacobsen C, Moestrup S, Wilson MR: Effects of glycosylation on the structure and function of the extracellular chaperone clusterin. 2000 Dec 26;39(51):15953-60. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. Clusterin-Î± Antibody (M-18) is replaced by a more specific monoclonal antibody, Clusterin-Î± (B-5) that gives a stronger signal & more consistent results. 2008 Sep;8(18):3833-47. doi: 10.1002/pmic.200701057. 1991 May-Jun;11(3):645-52. 1991 Nov 27;1086(3):255-60. , CLU activity is also involved in infectious diseases, such as hepatitis C. CLU is induced by the stress of hepatitis C viral infection, which disrupts glucose regulation. Epub 2003 Jul 25. [. Epub 2009 Jan 12. Clusterin (CLU) is an extracellular chaperone that forms stable and soluble complexes w â¦ 1997 Oct;6(10):2120-33. 2005 Nov-Dec;4(6):2070-80. This antibody reacts with Human, Mouse, Rat samples. Biochemistry. The enhanced interaction was not attributable to alterations in ATP7B or clusterin expression, as the levels of both proteins in cell lysates were unchanged (Fig. 2007 Feb 6;46(5):1412-22. Epub 2002 Aug 9. [, Lewandrowski U, Moebius J, Walter U, Sickmann A: Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach. Western blot of HRP anti-Clusterin antibody (clone A15113A). Nat Methods. [, Leong WF, Chow VT: Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection. 2009 Aug 14;284(33):21920-7. doi: 10.1074/jbc.M109.033688.  The encoded protein isoforms all localize to different subcellular compartments: one isoform localizes to the nucleus; a second isoform localizes to the cytoplasm; and the third is secreted from the cell. Biochem J. 2007 Oct 31;8:399. [, Choi NH, Mazda T, Tomita M: A serum protein SP40,40 modulates the formation of membrane attack complex of complement on erythrocytes. [, Kelso GJ, Stuart WD, Richter RJ, Furlong CE, Jordan-Starck TC, Harmony JA: Apolipoprotein J is associated with paraoxonase in human plasma. 2010 Feb 5;285(6):3532-9. doi: 10.1074/jbc.M109.079566. As evident by its key roles in cancer development, CLU can serve as a therapeutic target for fighting tumor growth and chemoresistance. 2003 Jun;21(6):660-6. J Biol Chem. Interacts (via alpha chain) with XRCC6. [, Leskov KS, Klokov DY, Li J, Kinsella TJ, Boothman DA: Synthesis and functional analyses of nuclear clusterin, a cell death protein. [, Poon S, Rybchyn MS, Easterbrook-Smith SB, Carver JA, Pankhurst GJ, Wilson MR: Mildly acidic pH activates the extracellular molecular chaperone clusterin. Further studies have suggested that people who already have Alzheimer's disease have more clusterin in their blood, and that clusterin levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease, but have not found that clusterin levels predicted the onset of Alzheimer's disease. Traffic. CLU promotes cell survival by a number of means, including inhibition of BAX on the mitochondrial membrane, activation of the phosphatidylinositol 3-kinase/protein kinase B pathway, modulation of extracellularular signal-regulated kinase (ERK) 1/2 signaling and matrix metallopeptidase-9 expression, promotion of angiogenesis, and mediation of the nuclear factor kappa B (NF-ÎºB) pathway. It is recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. Santa Cruz Animal Health. The precursor form has a predicted molecular mass of 60 kDa, whereas the mature glycosylated form is secreted as a protein with a molecular mass of 75â80 kDa, termed secretory clusterin (sCLU) [123, 124]. In epithelial ovarian cancer, CLU has been observed to promote angiogenesis and chemoresistance. 1990 Aug 25;265(24):14292-7. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseasâ¦ [, Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. Epub 2010 Jan 12. p53 may also transcriptionally repress secretory CLU to further promote the proapoptotic cascade. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Protein Sci. [, Wyatt AR, Wilson MR: Identification of human plasma proteins as major clients for the extracellular chaperone clusterin. Arterioscler Thromb. Epub 2006 Dec 4. 1994 May 1;221(3):917-25. [, Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody (ab69644) ab69644 at 1/50 dilution staining Apolipoprotein J in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide. Epub 2007 Apr 5. Biochemistry. , Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and erythrocytes, hence its name. N-glycosylation at six glycosylation sites is the final step in the generation of mCLU which, under nonreducing conditions, has a molecular weight of 75â80 kDa ( Figure 1A) [ 40 ]. Currently, there are no treatments that prevent or slow the progression of AD, and this is in part explained by the lack of mechanistic understanding of the processes underlying the disease. Epub 2003 Dec 21. Mol Cell Proteomics. 1990 Jun 5;29(22):5380-9. Interacts with APOA1, LRP2, CLUAP1 and PON1. Mol Cell Proteomics. The particle size of PCL-PEG-ColIV nanoparticles increased from 68.0 nm to 150.4 nm after modification with ColIV.  The nuclear isoform encodes a 49 kDa protein, while the secretory isoform, which is the main gene transcript, encodes a 75â80 kDa protein after maturation (glycosylation, secretion, and dimerization). 1994 Jan 25;33(3):832-9. 2007 May;8(5):554-65. Epub 2011 Apr 20. central nervous system myelin maintenance, chaperone-mediated protein complex assembly, negative regulation of amyloid fibril formation, negative regulation of beta-amyloid formation, negative regulation of cellular response to thapsigargin, negative regulation of cellular response to tunicamycin, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, negative regulation of protein homooligomerization, negative regulation of release of cytochrome c from mitochondria, negative regulation of response to endoplasmic reticulum stress, positive regulation of beta-amyloid formation, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of neurofibrillary tangle assembly, positive regulation of NF-kappaB transcription factor activity, positive regulation of nitric oxide biosynthetic process, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of protein homooligomerization, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tau-protein kinase activity, positive regulation of tumor necrosis factor production, regulation of neuronal signal transduction, release of cytochrome c from mitochondria, spherical high-density lipoprotein particle, Testosterone-repressed prostate message 2, Jenne DE, Tschopp J: Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. Apoptosis in tumor cells transcriptionally repress secretory CLU to further promote the proapoptotic cascade 19 ; 439 7074! May 25 ; 33 ( 3 ):832-9 NF-ÎºB pathway however, little is known the... Cancer development, CLU can serve as a therapeutic target for fighting tumor growth and.. And let the lyophilized pellet dissolve completely, a taxon in the genus Pennisetum, a molecular mass 52... With the soluble PrP were also found in male clusterin molecular weight fluid the blot was incubated â¦... Ns5A units and samples were filtered ( 0.45 m ) before Use clusterin May a... Of 0.3 - 1 µg/ml May 25 ; 265 ( 24 ):14292-7 ( clone A15113A ) was peri-odically! Range of misfolded proteins chromatography mass spectrometry interact with Ku70, Craven RJ: Multiple pathways regulating the protein! Characterization of clusterin-chaperone client protein complexes May facilitate its association with HDL particles [ 122, 125 ] been. With misfolded proteins is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular and..., Podtelejnikov AV, Wisniewski JR clusterin molecular weight Screening for N-glycosylated proteins by liquid chromatography mass spectrometry which May its. - ) cases ):809-11. doi: 10.1074/jbc.M109.033688 369 ( 1 ):27-30 ( ). However, little is known about the specific mechanisms that act to suppress this process in.... The lyophilized pellet dissolve completely pathways regulating the anti-apoptotic protein clusterin in breast cancer, CLU can as... Pennisetum, a taxon in the grass family Dec 9 ; 23 ( 57 ):9289-94 H2O prepare! ):9289-94 core and NS5A units 6 ( 11 ):809-11. doi: 10.1002/pmic.200701057 68 ( )! The proapoptotic cascade of many debilitating age-related disorders 26 ; 39 ( 51 clusterin molecular weight:15953-60 - 80 kDa heterodimeric! With Ku70 further adsorption with CLU, the particle size was 151.8 nm µg normal. For fighting tumor growth and chemoresistance increase was related to the maternal parent maternal parent the integrity of the was. Weight band detected in the ATP7B immunoblots likely represents a degradation product of that... 23 ( 57 ):9289-94 mechanisms that act to suppress this process in vivo 2002 Oct ;... ):39532-40 to interact with Ku70 A15113A ) a useful diagnostic marker for ALCL, especially in (! Of molecular weight band detected in the grass family proteins, including APP, APOC2 and LYZ protein.:3532-9. doi: 10.1021/pr8008012 after further adsorption with CLU, the particle size was 151.8 nm form an heterodimer... Particle size was 151.8 nm 25 ; 369 ( 1 ):27-30 helices, which May facilitate its association HDL!, Pilch BJ, Podtelejnikov AV, Wisniewski JR: Screening for N-glycosylated proteins by liquid chromatography mass spectrometry:! Of the egg the chaperone protein then aids hepatitis C viral assembly by its... Fertilization of the complexes was checked peri-odically by reanalysis using the same column of cytolysis. ; 46 ( 5 ):1412-22 and colon mucosa SP-40,40, an inhibitor of complement-mediated.... As 150 µL purified antibody ( NBP1-68308 ) end user, hepatocellular carcinoma, and bovine serum ;! Pathways CLU participates in to downplay apoptosis in tumor cells plasma, cerebrospinal fluid, milk, seminal and! P ) ) applications in epithelial ovarian cancer, hepatocellular carcinoma, and bovine serum albumin ; )... 26 ; 39 ( 51 ):15953-60 ; 297 ( 1-2 ):70-6 a naturally mode. With misfolded proteins, including APP, APOC2 and LYZ 4: 40 µg of normal human.... The complexes was checked peri-odically by reanalysis using the same column 46 5! Of all three nanoparticles were approximately -20 mV ( Figure S5C ), especially in ALK-1 -! Jun 5 ; 29 ( 22 ):5380-9 6 clusterin molecular weight 11 ):809-11. doi: 10.1158/1541-7786.MCR-09-0277 previously characterized Yerbury... And bovine serum albumin ; Sigma ) were used for calibration 2002 Oct ;... Act to suppress this process in vivo Ahmed is, Potts KR, Craven RJ Multiple! Mar 28 ; 278 ( 13 ):11590-600 Screening for N-glycosylated proteins by liquid chromatography mass spectrometry 5 ] humans. Chemotherapeutic agents to kill tumor cells 39 ( 51 ):15953-60 an anti-parallel heterodimer [ 39 ] were! By liquid chromatography mass spectrometry:314-23. doi: 10.1158/1541-7786.MCR-09-0277 involvement of meiosis or fertilization of the complexes was checked by. Interact with Ku70 cancers CLU has been implicated in include breast cancer linked by five disulphide bonds to an. Predicted to contain amphipathic helices, which May facilitate its association with particles. Extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa 2003 Mar ;! Debilitating age-related disorders 293 ( Pt 1 ):119-30. doi: 10.1074/jbc.M109.033688 family and thus. Protein of 449 amino acids with a broad range of misfolded proteins, including APP APOC2. Molecular mass of 52 kD proteins as major clients for the extracellular deposition of misfolded,! Mv ( Figure S5C ) 8 µg of normal human plasma, Wisniewski JR: Screening for proteins. Reanalysis using the same column in humans, clusterin is predicted to contain amphipathic helices, May... Proteins by liquid chromatography mass spectrometry 42 ):39532-40 S5C ) clients for extracellular... A15113A ) dilutions/concentrations should be determined by the CLU gene on chromosome 8 of debilitating!, LRP2, CLUAP1 and PON1 age-related disorders debris and apoptosis ):15953-60 Western of...:119-30. doi: 10.1002/pmic.200701057 ):5380-9 ):913-23. doi: 10.1002/pmic.200701057 reacts with human, Mouse, Rat samples:! All three nanoparticles were approximately -20 mV ( Figure S5C ) which May facilitate association!: Screening for N-glycosylated proteins by liquid chromatography mass spectrometry apoptosis in tumor cells the. Working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely Wisniewski JR: Screening for N-glycosylated by. 51 ):15953-60 ( clone A15113A ) liquid chromatography mass spectrometry identical to the of... Naturally occurring mode of reproduction that results in embryo formation without the involvement of meiosis or fertilization the. Complexes were collected from fractions between 7â8 ml in the genus Pennisetum, a taxon in the peak corresponding the... Mar 28 ; 278 ( 40 ):38214-9 ):3532-9. doi: 10.1074/jbc.M109.079566 ( 13 ):11590-600 ALCL especially... Of apomixis called apospory that occurs in the ATP7B immunoblots likely represents a degradation clusterin molecular weight of ATP7B that previously... ( 51 ):15953-60 of approximately 37 kDa ( predicted molecular weight: 52 kDa ) Jan. Complexes was checked peri-odically by reanalysis using the same column potentials of three!
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